Restriction Enzymes
New England Biolabs, Inc. Endo Hf – 500000 units
Endo Hf is a recombinant protein fusion of Endoglycosidase H and maltose binding protein. Endo Hf cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins equally as well as Endo H.
New England Biolabs, Inc. PspGI – 1000 units
A restriction endonuclease that recognizes the sequence ^CCWGG_.
New England Biolabs, Inc. HpyCH4V – 100 units
A restriction endonuclease that recognizes the sequence TG_^CA.
New England Biolabs, Inc. PciI – 200 units
A restriction endonuclease that recognizes the sequence A^CATG_T.
New England Biolabs, Inc. AleI-v2 – 2500 units
AleI-v2 is an engineered and improved version of AleI that recognizes the sequence CACNN^NNGTG.
New England Biolabs, Inc. HinfI – 25000 units
A restriction endonuclease that recognizes the sequence G^ANT_C.
New England Biolabs, Inc. MspA1I – 500 units
A restriction endonuclease that recognizes the sequence CMG_^CKG.
New England Biolabs, Inc. PstI – 10000 units
A restriction endonuclease that recognizes the sequence C_TGCA^G.
New England Biolabs, Inc. BceAI – 50 units
A restriction endonuclease that recognizes the sequence ACGGCNNNNNNNNNNNN^NN_.
New England Biolabs, Inc. Hpy99I – 100 units
A restriction endonuclease that recognizes the sequence _CGWCG^.
New England Biolabs, Inc. EagI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GGCC_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I FLTRD-25MG
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
New England Biolabs, Inc. EnGen Spy dCas9 (SNAP-tag) – 400 pmol
EnGen Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity. EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.