Restriction Enzymes
New England Biolabs, Inc. EcoRI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
Agilent Technologies BRIL MULTIPLEX QPCR MST MIX
Amplifies up to 4 targets in a single real-time QPCR reaction.
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 1000U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
Apexbio Technology LLC Dpn I(Synonyms: DpnI, DpnI Restriction Enzyme, Dam methylation-sensitive restriction enzyme DpnI), 500U.
Dpn I is a restriction endonuclease sensitive to Dam methylation specifically recognizing and cleaving the adenine N6-methylated sequence GA/TC within DNA Cleavage is dependent on adenine methylation status mammalian CpG methylation overlapping Dpn I sites blocks cleavage and methylation by Dcm is not recognized by this enzyme It is routinely used for the rapid digestion of plasmid DNA PCR fragments and genomic DNA and finds broad utility in genetic typing molecular cloning Southern blotting SNP genotyping and restriction fragment length polymorphism (RFLP) analyses
New England Biolabs, Inc. BspQI HF-500 units
A Type IIS restriction endonuclease that recognizes the sequence GCTCTTC(1/4). An E. coli strain that carries the modified cloned BspQI gene from Bacillus sphaericus. BspQI-HF is an engineered and improved version of BspQI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)
New England Biolabs, Inc. PaqCI - 1000 units
PaqCI is a Type IIS restriction enzyme with a 7 basepair recognition sequence: CACCTGC(4/8). It is sourced from an E. coli strain that carries the modified cloned PaqCI gene from Paucibacter aquatile. PaqCI requires multiple sites for efficient cleavage -- PaqCI Activator is included for optimal cleavage. This enzyme is supplied with NEB's rCutSmart Buffer and is heat inactivated.
New England Biolabs, Inc. HaeIII – 15000 units
A restriction endonuclease that recognizes the sequence GG_^CC.
New England Biolabs, Inc. BglI – 10000 units
A restriction endonuclease that recognizes the sequence GCCN_NNN^NGGC.
New England Biolabs, Inc. HpaI – 500 units
A restriction endonuclease that recognizes the sequence GTT_^AAC.
New England Biolabs, Inc. ClaI – 1000 units
A restriction endonuclease that recognizes the sequence AT^CG_AT.
New England Biolabs, Inc. AlwI – 500 units
A restriction endonuclease that recognizes the sequence GGATCNNNN^N_.
New England Biolabs, Inc. AccI – 5000 units
A restriction endonuclease that recognizes the sequence GT^MK_AC.